目的 建立同时测定人参首乌胶囊中8种人参皂苷类成分含量的高效液相色谱-蒸发光散射器检测法。方法 UltimateC18(4.6 mm×250 mm,5 μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速1.0 mL·min-1,蒸发光散射检测器漂移管温度100 ℃,气体流量2.5 L·min-1,增益2。结果 人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd、20(S)-Rg3分别在0.483~12.1、0.444~11.1、0.502~12.6、0.490~12.3 、0.455~23.2、0.464~11.6、0.264~13.2和0.244~12.2 μg内成良好的线性关系(r > 0.999 5),平均加样回收率分别为101.9%、104.5%、98.3%、97.9%、97.8%、101.2%、100.6%和99.0%。结论 该方法简便、准确、分离效果好,无干扰,可用于人参首乌胶囊的质量评价。
Abstract
To develop an HPLC-ELSD method for simultaneous determination of the contents of eight ginsenosides in Renshenshouwu capsules(traditional Chinese medicines). METHODS The analysis was performed on an Ultimate C18 column(4.6 mm× 250 mm,5 μm) eluted with mobile phase consisting of acetonitrile and water in gradient mode at a flow rate of 1.0 mL·min-1. The column temperature was set at 30 ℃. The ELSD drift tube temperature was set at 100 ℃,and the gas flow rate was set at 2.5 L·min-1. RESULTS Good linearity(r>0.999 5) was obtained in the range of 0.483-12.1 μg,0.444-11.1 μg,0.502-12.6 μg,0.490-12.3 μg,0.455-23.2 μg,0.464-11.6 μg,0.264-13.2 μg and 0.244-12.2 μg for ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1,ginsenoside Rc,ginsenoside Rb2,ginsenoside Rb3,ginsenoside Rd and 20(S)- ginsenoside Rg3,respectively. The average recoveries of the eight compounds were 101.9%,104.5%,98.3%,97.9%,97.8%,101.2%,100.6% and 99.0 %,respectively. CONCLUSION The method is reliable,simple,precise, and can be used for the quality control of Renshenshouwu capsules.
关键词
人参首乌胶囊 /
人参皂苷 /
高效液相色谱-蒸发光散射器检测法 /
含量测定
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Key words
Renshenshouwu capsules /
ginsenoside /
HPLC-ELSD /
assay
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中图分类号：
R284
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参考文献
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脚注
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基金
国家“重大新药创制”科技重大专项资助项目(2012ZX09103-101-057);国家自然科学基金资助项目(21175031,20905019)
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